GC Content & Nucleotide Composition
Calculate GC percentage and base counts (A, C, G, T, U) for DNA or RNA. Identify sequence complexity, contamination, or bias in seconds.
Drop file here
Drag & drop supported
| Record | Length | A | C | G | T | U | N/Other | GC % |
|---|
Why GC Content Matters
The GC content (percentage of Guanine and Cytosine bases) is a critical metric in genetics. High GC content generally indicates higher melting temperatures due to the triple hydrogen bonds between G and C.
Calculation Method
This tool counts every nucleotide. Formula: GC% = (G + C) / (A + C + G + T + U) * 100. Ambiguous characters (N, R, Y, etc.) are excluded from GC
percentage calculation.
Use Cases
- •Estimating melting temperature (Tm) for PCR primers.
- •Detecting contamination from organisms with different GC profiles.